| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.100501 |
| Chromosome: | chromosome 9 |
| Location: | 4897014 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397808 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGCGCTCTGGAGCTCGGCGGCGGTGTC |
| Internal bar code: | TCGGCCTGTCTGAGTACCAGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 615 |
| LEAP-Seq percent confirming: | 98.0339 |
| LEAP-Seq n confirming: | 3640 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTCTGGACCCTCTCCAT |
| Suggested primer 2: | CCTGAGGAGTAAGGACGCAG |