Insertion junction: LMJ.RY0402.100533_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.100533
Chromosome:chromosome 16
Location:6083863
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g676000 (1 of 102) PF01753 - MYND finger (zf-MYND) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCATCTGCCGCTGCGTCTGCTTCTGGCGC
Internal bar code:TGTGACTCAATTTAGAATCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1051
LEAP-Seq percent confirming:99.0712
LEAP-Seq n confirming:320
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AACGTATGGACAGGGCAGAC
Suggested primer 2:GGACATTGAAGTGGGTCTCC