| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.100538 |
| Chromosome: | chromosome 7 |
| Location: | 4133768 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342750 | (1 of 1) K18106 - D-galacturonate reductase (GAAA) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGCGCATTGTTGTTCTTGTGGCGGTTGA |
| Internal bar code: | GTCAACACCCTACATAGCACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 99.1135 |
| LEAP-Seq n confirming: | 3354 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAACAGTGGCAATGAGTT |
| Suggested primer 2: | ACTTCTCCGCTCCTTCTTCC |