| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100555 |
| Chromosome: | chromosome 7 |
| Location: | 3409085 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335226 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGCAAGTGGCGCCGGGCTTGGTGGCTC |
| Internal bar code: | CGCCCCATCTGCTGTCCCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 440 |
| LEAP-Seq percent confirming: | 99.4223 |
| LEAP-Seq n confirming: | 20998 |
| LEAP-Seq n nonconfirming: | 122 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCGGGACCTCCCTCT |
| Suggested primer 2: | CTCAGGGCTAGGTGGTTCTG |