Insertion junction: LMJ.RY0402.100567_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.100567
Chromosome:chromosome 17
Location:558395
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g699750 CTL2 (1 of 1) IPR016187//IPR024616 - C-type lectin fold // Pherophorin; Putative pherophorin with C-type lectin 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTACAATTACAGTGACTGAGGACTTCCA
Internal bar code:CGTAGTTGTACGCCCAGAGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:413
LEAP-Seq percent confirming:99.6805
LEAP-Seq n confirming:2496
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGACGTTAGGCGTCATCATT
Suggested primer 2:ACGATATATGCTATGCCGCC