| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.100572 |
| Chromosome: | chromosome 9 |
| Location: | 1306258 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGACCTCCTCCTGTGGCACACACCGAG |
| Internal bar code: | GACATTCAGGGATGTCATAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 980 |
| LEAP-Seq percent confirming: | 99.2861 |
| LEAP-Seq n confirming: | 1947 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGCTCCTTCCACACCTCG |
| Suggested primer 2: | TTACCAAGTCGCAAATGCTG |