| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100578 |
| Chromosome: | chromosome 4 |
| Location: | 3435073 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g227650 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCCTTCAGAAAAATGTAAGCTAGTGTG |
| Internal bar code: | GGAATCCGCGCGTGCTATGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 251 |
| LEAP-Seq percent confirming: | 99.6028 |
| LEAP-Seq n confirming: | 4263 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGACTCGCCACCTCTTC |
| Suggested primer 2: | ATGGCTGACTCCATTGTTCC |