| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100595 |
| Chromosome: | chromosome 8 |
| Location: | 1794432 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g366450 | (1 of 2) PTHR23213:SF30 - DISHEVELLED ASSOCIATED ACTIVATOR OF MORPHOGENESIS, ISOFORM D | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCTGCACTTCGTTCCCCCAACCTAAGCA |
| Internal bar code: | TGCGAGCCTTCTGATGCGTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 950 |
| LEAP-Seq percent confirming: | 99.8264 |
| LEAP-Seq n confirming: | 575 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGCACGTCATCTACCCT |
| Suggested primer 2: | GACGAGCTGATGAGTAGGGC |