| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.100597 |
| Chromosome: | chromosome 2 |
| Location: | 7842912 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141950 | MOT19 | (1 of 5) PF03765 - CRAL/TRIO, N-terminal domain (CRAL_TRIO_N); Predicted protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGGTGCCGACCTCCCCTGCCGCCGCGT |
| Internal bar code: | TTCCGGGCTCTTCCGCTCTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGTTTGAGATGCCGCTA |
| Suggested primer 2: | TTGTAATCTGGAAGTCCGCC |