| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100604 |
| Chromosome: | chromosome 9 |
| Location: | 5147730 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398993 | HPD1 | (1 of 3) 1.13.11.27 - 4-hydroxyphenylpyruvate dioxygenase / p-hydroxyphenylpyruvate oxidase; 4-hydroxyphenylpyruvate dioxygenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTCAGCACTGAGGTAAGGCTTGGACAC |
| Internal bar code: | GGTGCGTGATTTGGAGTATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1175 |
| LEAP-Seq percent confirming: | 99.3769 |
| LEAP-Seq n confirming: | 1595 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGTACGTGTGCCTACCT |
| Suggested primer 2: | CTCGTTCTGCTCCAGGTAGG |