| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.100610 |
| Chromosome: | chromosome 1 |
| Location: | 3398924 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGTGCATGACATCCGGAAGCACTTGAG |
| Internal bar code: | AAGCTGCCGCTAAAACGGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 218 |
| LEAP-Seq percent confirming: | 40.4575 |
| LEAP-Seq n confirming: | 4068 |
| LEAP-Seq n nonconfirming: | 5987 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCCACATATGCAGCAAGC |
| Suggested primer 2: | TAGTCAAGTCGGTGCTGACG |