| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100613 |
| Chromosome: | chromosome 14 |
| Location: | 3112923 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628702 | (1 of 1) PF11919 - Domain of unknown function (DUF3437) (DUF3437) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTTTACTTTCGCTGGCATCTACAACCC |
| Internal bar code: | TTGGGACAACTTCAGCACAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 29 |
| LEAP-Seq percent confirming: | 94.6429 |
| LEAP-Seq n confirming: | 106 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCACAACCAAACAGTCC |
| Suggested primer 2: | CTCTTCCCTGCGATTTTCAC |