Insertion junction: LMJ.RY0402.100614_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.100614
Chromosome:chromosome 6
Location:4363987
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278268 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCATGCCCCACCCAGCAGCCCTTACCA
Internal bar code:GGCCTTATTGCGCAACGGAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:721
LEAP-Seq percent confirming:97.7107
LEAP-Seq n confirming:2817
LEAP-Seq n nonconfirming:66
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TATCTGCCGTTGTGCTTCTG
Suggested primer 2:GAAGCAGCAAGCCAGGTAAC