| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100625 |
| Chromosome: | chromosome 2 |
| Location: | 2464198 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091900 | FAP301 | Flagellar Associated Protein 301 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACCACGCCTACCTCACGCCATTTCTAAC |
| Internal bar code: | CTGATAATATCATAACATCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 184 |
| LEAP-Seq percent confirming: | 99.6012 |
| LEAP-Seq n confirming: | 2248 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCAGCTCACTTGCATCC |
| Suggested primer 2: | CTAGACCAGACGGCTTGAGG |