| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.100630 |
| Chromosome: | chromosome 14 |
| Location: | 1645443 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619000 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGCTTTGTCCCTAGTCCTCCTCGCACAT |
| Internal bar code: | CCGAGGGATCCTTTCGGGCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 623 |
| LEAP-Seq percent confirming: | 99.6681 |
| LEAP-Seq n confirming: | 6306 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTGTTCCGTGTTGGGTCT |
| Suggested primer 2: | AAAACAGCGGACGGTTATTG |