Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.100656 |
Chromosome: | chromosome_12 |
Location: | 6145962 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g536050 | ALA1 | Phospholipid-transporting ATPase | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACGTCTTCCGCCAACATGCACGAATGTCTT |
Internal bar code: | CATCGTGCGTCGCGGACGAAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 54 |
LEAP-Seq percent confirming: | 77.5947 |
LEAP-Seq n confirming: | 471 |
LEAP-Seq n nonconfirming: | 136 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCTGAGAATGTAGCCTTGC |
Suggested primer 2: | CTTCTGCATCTCACGGATGA |