| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100668 |
| Chromosome: | chromosome 11 |
| Location: | 3413773 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g480750 | (1 of 1) 3.4.19.5//3.5.1.1 - Beta-aspartyl-peptidase // Asparaginase / L-asparagine amidohydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCGCAGCTCCCCTCAGACCCACCTGATC |
| Internal bar code: | CACTACCGCGAACGGAACCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 381 |
| LEAP-Seq percent confirming: | 98.7603 |
| LEAP-Seq n confirming: | 2629 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACCACACCACAGACCACG |
| Suggested primer 2: | AGGAAGGGAAGCTGGAGGTA |