| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.100670 |
| Chromosome: | chromosome 13 |
| Location: | 1409119 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572000 | TPM1,UPM2,SUMT2,COBA | Putative tetrapyrrole methylase; (1 of 1) 2.1.1.198 - 16S rRNA (cytidine(1402)-2'-O)-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAGCCCGCCGGGCAGGGGCTGCTGGGCC |
| Internal bar code: | TTGGCCGGTTCAACTATCTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 277 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGCGCAATCTCCTACTTC |
| Suggested primer 2: | CGTCATGGGAAGGCTGTATT |