| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100674 |
| Chromosome: | chromosome 5 |
| Location: | 644014 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g231050 | (1 of 1) K10636 - autocrine motility factor receptor (AMFR, GP78) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCAGGCCCTAGTGGGCCAGCCGGTGCTT |
| Internal bar code: | GATGGAATGGACTCCTCATGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 798 |
| LEAP-Seq percent confirming: | 99.7235 |
| LEAP-Seq n confirming: | 3607 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCATCTTGTGCAGAACTCG |
| Suggested primer 2: | CTCCCCTCTGCAACATCATT |