| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100703 |
| Chromosome: | chromosome 6 |
| Location: | 3996283 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278198 | (1 of 1) 1.14.11.33//4.2.99.18 - DNA oxidative demethylase / Alkylated DNA repair protein // DNA-(apurinic or apyrimidinic site) lyase / Phage-T4 UV endonuclease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCGTGCGGCCATGGTCTCTCGTTGGGC |
| Internal bar code: | GGGGAGACGACCATTAGTCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 440 |
| LEAP-Seq percent confirming: | 99.8753 |
| LEAP-Seq n confirming: | 801 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTGACTGCTCCTTTGAG |
| Suggested primer 2: | CTGCAGCCGATACAGACAAA |