| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100703 |
| Chromosome: | chromosome 12 |
| Location: | 4139277 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518107 | (1 of 1) PF04182 - B-block binding subunit of TFIIIC (B-block_TFIIIC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGAGGGCCGTGGGGCTGGGGGCGGCGGT |
| Internal bar code: | AGCGGCATGAGCAGTACTGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 412 |
| LEAP-Seq percent confirming: | 99.1158 |
| LEAP-Seq n confirming: | 6950 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACCTGTGTGTTGCTGCT |
| Suggested primer 2: | GCCAGGGATATCTGTGAGGA |