| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100726 |
| Chromosome: | chromosome 13 |
| Location: | 1105319 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569275 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATTGCAACACACGGGCACGTTACGTTGC |
| Internal bar code: | GGGTCGGGAGGGCAACCCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 555 |
| LEAP-Seq percent confirming: | 98.6616 |
| LEAP-Seq n confirming: | 12605 |
| LEAP-Seq n nonconfirming: | 171 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCAGGCCCACAGTTACAG |
| Suggested primer 2: | CGGACCGCTTTGAATGTACT |