| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100742 |
| Chromosome: | chromosome 6 |
| Location: | 4831565 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278350 | AGD1 | (1 of 1) 1.3.1.78 - Arogenate dehydrogenase (NADP(+)) / TyrAAT2; Arogenate/prephenate dehydrogenase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCACTGATTAAACAAGTATGCTTAGGCG |
| Internal bar code: | GTTGTCTACTCTGTGCCTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1450 |
| LEAP-Seq percent confirming: | 99.5724 |
| LEAP-Seq n confirming: | 1630 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATTGCGAGGAAGATAAA |
| Suggested primer 2: | ACCCAGCCATTTTGACAGAC |