| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100742 |
| Chromosome: | chromosome 6 |
| Location: | 4831567 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278350 | AGD1 | (1 of 1) 1.3.1.78 - Arogenate dehydrogenase (NADP(+)) / TyrAAT2; Arogenate/prephenate dehydrogenase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTACCTAAAAAGAAACACTATAAAGGTA |
| Internal bar code: | CAGGTGTAGTGGCACCCCACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 707 |
| LEAP-Seq percent confirming: | 98.0299 |
| LEAP-Seq n confirming: | 4976 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATTGCGAGGAAGATAAA |
| Suggested primer 2: | ACCCAGCCATTTTGACAGAC |