| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.100757 |
| Chromosome: | chromosome 6 |
| Location: | 6956324 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g296250 | SYK1,TSK2 | Putative organellar lysyl-tRNA synthetase, tRNA synthetase class II (D, K and N) family protein; (1 of 1) PTHR22594//PTHR22594:SF4 - ASPARTYL/LYSYL-TRNA SYNTHETASE // LYSINE--TRNA LIGASE-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGACCCCCGCGGGTGCGACAGCATTTCC |
| Internal bar code: | CAGCGCCCGGCAGTGAGAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 99.1808 |
| LEAP-Seq n confirming: | 1695 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACATCAACGCCAACATCA |
| Suggested primer 2: | GAGGTGTGGTGAAAGGGAGA |