| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.100761 |
| Chromosome: | chromosome 2 |
| Location: | 5042453 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104550 | Paf1 complex subunit CDC73; (1 of 1) K15175 - parafibromin (CDC73) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGAGGCGGCCGCTCCCATCTCCTGCCA |
| Internal bar code: | CAGAGGGCAAATATGATGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 99.4675 |
| LEAP-Seq n confirming: | 8406 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATCACGTGTGGTGTATG |
| Suggested primer 2: | CTGCACACGTCATTTCGACT |