Insertion junction: LMJ.RY0402.100762_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.100762
Chromosome:chromosome 9
Location:1697246
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g396950 MFT25,CGL15,PHT4I,PHT4-9 Na+-dependent inorganic phosphate cotransporter; (1 of 4) K08193 - MFS transporter, ACS family, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), other (SLC17A) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGTGGCCACGCATGTCATCCGCGATTTG
Internal bar code:CGTATGGCAATATGTTGAGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:474
LEAP-Seq percent confirming:99.7429
LEAP-Seq n confirming:9310
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACACGGTACGAGCTTGTGTG
Suggested primer 2:GTCACATCACACAACGAGGG