| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100808 |
| Chromosome: | chromosome 2 |
| Location: | 5438243 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107600 | CGL118,GAK1 | UDP-D-glucuronate kinase; (1 of 1) 2.7.1.43 - Glucuronokinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCTGTGAACCTGGGATCAGGCAAAATA |
| Internal bar code: | TGACGACTCCTCCTGTTGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 87.5936 |
| LEAP-Seq n confirming: | 1638 |
| LEAP-Seq n nonconfirming: | 232 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGCAAGTAGCTTTACGG |
| Suggested primer 2: | ATTCGCACGCGCTCTAGTAT |