| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100855 |
| Chromosome: | chromosome 12 |
| Location: | 3876410 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515650 | EIF3K | Eukaryotic translation initiation factor 3, subunit K; (1 of 1) K15028 - translation initiation factor 3 subunit K (EIF3K) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGACAACCAGTCGGTCGTGAAGCGCACC |
| Internal bar code: | TTCTAGTTCGTGTAGTTGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 147 |
| LEAP-Seq percent confirming: | 99.6721 |
| LEAP-Seq n confirming: | 1216 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTAAGCGCCTCTAACCC |
| Suggested primer 2: | GAATCAGAGGCAACAGAGGC |