| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.100876 |
| Chromosome: | chromosome 7 |
| Location: | 1946901 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325749 | (1 of 1) PF14924 - Protein of unknown function (DUF4497) (DUF4497) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTAGCGAGGGGGTGGAGGGAGTGGGGC |
| Internal bar code: | AAGCGATGCGTGAGTCTGTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 98.6111 |
| LEAP-Seq n confirming: | 497 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCTGAACAAGACAAGACG |
| Suggested primer 2: | TCGGAAATCATTCCTCCTTG |