Insertion junction: LMJ.RY0402.100878_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.100878
Chromosome:chromosome 7
Location:6334567
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g357200 UGD1,EZY12 Similar to UDP-Glucose Dehydrogenase; (1 of 3) 1.1.1.22 - UDP-glucose 6-dehydrogenase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGCTTGGACGCGTGACCACGTGTTGACA
Internal bar code:GTGAATTGTGCGACTTGCGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:238
LEAP-Seq percent confirming:99.8618
LEAP-Seq n confirming:1445
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TATGTTGAGTGCGGAGCTTG
Suggested primer 2:AGATCGCTGTGTACGGCTTT