| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100890 |
| Chromosome: | chromosome 17 |
| Location: | 3580294 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725650 | NBS | (1 of 2) K03453 - bile acid:Na+ symporter, BASS family (TC.BASS); Sodium:bile acid symporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCAAAGAAAGCACGACCCAGAACCACTG |
| Internal bar code: | ATTGTCGGTGCTAACGACATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 605 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 200 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGACCCAGTGGTGACAAA |
| Suggested primer 2: | CCGTGTGTGTGTGTGTGTGT |