| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100897 |
| Chromosome: | chromosome 15 |
| Location: | 430395 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636300 | CPN2 | Tubulin folding cofactor B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCACGCCCTGCTGTCGTATGCCTCGTC |
| Internal bar code: | ACCGCCTAGTTCCCCAAGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 731 |
| LEAP-Seq percent confirming: | 77.907 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCCCAAACTAAACCAAA |
| Suggested primer 2: | TGCGGTTGTGGTGAAAATTA |