Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.100904 |
Chromosome: | chromosome 10 |
Location: | 910447 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g424300 | KIN16A,KIN12-2,KIN16-1 | (1 of 32) 3.6.4.4 - Plus-end-directed kinesin ATPase / Kinesin; Kinesin motor protein | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTATCTATTTTTTTGTATAGGCATCACC |
Internal bar code: | GGAAAACGGCTATTCAAGTTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 742 |
LEAP-Seq percent confirming: | 99.6805 |
LEAP-Seq n confirming: | 1872 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCCTATTGCAGGCACAGTT |
Suggested primer 2: | CACTGAAGCATTCGACAGGA |