| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.100919 |
| Chromosome: | chromosome 5 |
| Location: | 1648573 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243150 | Carbonic anhydrase-related protein; (1 of 1) PF04357 - Family of unknown function (DUF490) (DUF490) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGGGGGGTGGGGTGGGGGTTATGGGCG |
| Internal bar code: | GGCACATGAGAGATCGCTACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 95.7812 |
| LEAP-Seq n confirming: | 613 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACACCGGACTACACACAC |
| Suggested primer 2: | ACCCCTTCATAGACATTGCG |