Insertion junction: LMJ.RY0402.100928_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.100928
Chromosome:chromosome 17
Location:1525554
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g707250 MFT29 Major facilitator superfamily transporter; (1 of 82) IPR020846 - Major facilitator superfamily domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCCATCTACCCGCTGCAGAAGTCCATCC
Internal bar code:GGGAAGGTGTACGGAAAGGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:267
LEAP-Seq percent confirming:98.2249
LEAP-Seq n confirming:3984
LEAP-Seq n nonconfirming:72
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TCCCTTCCCTTGTAAATCCC
Suggested primer 2:TGGTGGGGAAAGATAAGTGC