Insertion junction: LMJ.RY0402.100943_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.100943
Chromosome:chromosome_7
Location:1797631
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g325724 PTP1 Protein tyrosine phosphatase antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):GGCTGTGCAGGCCAGGGCAGGGTGGGGAAG
Internal bar code:TGGCGGGCAAAGATATCTGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1150
LEAP-Seq percent confirming:99.7248
LEAP-Seq n confirming:5073
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAGAAGACGAAGAAGCAG
Suggested primer 2:ACACCTAATACTGCGGCACC