Insertion junction: LMJ.RY0402.100948_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.100948
Chromosome:chromosome 9
Location:7412686
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g413250 GT90-15,GT90F15 GT90 family protein 15; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAGCTGCGGCGCCTGGACGCGGAGGAGC
Internal bar code:GGGAAAACGAGGTTGGGAGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:616
LEAP-Seq percent confirming:99.2771
LEAP-Seq n confirming:1648
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAACATCTTTGCAACCCT
Suggested primer 2:AGGTGGTTGTAACCGACAGC