| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.100965 |
| Chromosome: | chromosome 11 |
| Location: | 2388036 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g474750 | (1 of 1) 2.4.2.39 - Xyloglucan 6-xylosyltransferase / Xyloglucan 6-alpha-D-xylosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAACCCGCCGTGAAAGCTTGAGGAGGTT |
| Internal bar code: | CGAAACATAGTGGACTCCGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 671 |
| LEAP-Seq percent confirming: | 99.4596 |
| LEAP-Seq n confirming: | 3497 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGACTGGTGGTACTCGT |
| Suggested primer 2: | GTTGTCACCGGACTTGGAGT |