| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.100966 |
| Chromosome: | chromosome 12 |
| Location: | 5942170 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534400 | (1 of 18) IPR011051 - RmlC-like cupin domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCGCCAACGGCCACGCGGTACCTGCGCA |
| Internal bar code: | GTTCATTTAGGTCACCCCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1091 |
| LEAP-Seq percent confirming: | 98.6755 |
| LEAP-Seq n confirming: | 1043 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTACAGCGACTTCAAGC |
| Suggested primer 2: | GCCCTGATTGTAGCGTTGAT |