| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100991 |
| Chromosome: | chromosome 6 |
| Location: | 7433526 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299900 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTTGAGCAGCGTCCCAGCACCGTCGTC |
| Internal bar code: | TATCACCTTGGGTCGACTGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 653 |
| LEAP-Seq percent confirming: | 98.8033 |
| LEAP-Seq n confirming: | 1899 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGAGTTCTACCCCAGCAG |
| Suggested primer 2: | GACCTGTTGCAATTCCTGGT |