| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.101001 |
| Chromosome: | chromosome 6 |
| Location: | 8529855 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g308050 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCTCTTTAGCAGTGACAGCAGCCCCTG |
| Internal bar code: | CCGAGTATGCAACCAAATTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1039 |
| LEAP-Seq percent confirming: | 99.7264 |
| LEAP-Seq n confirming: | 6561 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGCGCATGTATTGCAGTG |
| Suggested primer 2: | GCTGGCAGAGTGTTGTCAAG |