| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.101016 |
| Chromosome: | chromosome 16 |
| Location: | 2656464 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661976 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGCTTTTTGCGGTGACGGAGGCTAACG |
| Internal bar code: | CCATCACTGTTCAGAGCCAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 96.25 |
| LEAP-Seq n confirming: | 462 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAGGGAGCACAGCTACA |
| Suggested primer 2: | GACAAGTCCGGTTGCGTAAT |