| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.101019 |
| Chromosome: | chromosome 17 |
| Location: | 1802125 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709550 | (1 of 2) K06709 - jumonji domain-containing protein 2 [EC:1.14.11.-] (JMJD2, JHDM3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGAGGGGGCGGAGGAGGCCGAGGCTAA |
| Internal bar code: | TATTTACTGGCGCGTTGTATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 215 |
| LEAP-Seq percent confirming: | 95.3221 |
| LEAP-Seq n confirming: | 2649 |
| LEAP-Seq n nonconfirming: | 130 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACCAGGTAGAAGAAGCG |
| Suggested primer 2: | GGCCTTTATTACGCACCTGA |