| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.101019 |
| Chromosome: | chromosome 17 |
| Location: | 1802131 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709550 | (1 of 2) K06709 - jumonji domain-containing protein 2 [EC:1.14.11.-] (JMJD2, JHDM3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCCTCCTCGTCGCTGCTCTGGTCAGCG |
| Internal bar code: | ACGCGTGGGCGGTTAACAGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 64 |
| LEAP-Seq percent confirming: | 65.1515 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGCGCTTGCTCGTGT |
| Suggested primer 2: | GGCCTTTATTACGCACCTGA |