| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.101048 |
| Chromosome: | chromosome 3 |
| Location: | 8360302 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g198750 | (1 of 2) K02327 - DNA polymerase delta subunit 1 (POLD1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACACTCCTCAGGTGTTGTACAACGCCC |
| Internal bar code: | AGCCGGGCTACTGATCGCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 586 |
| LEAP-Seq percent confirming: | 98.9576 |
| LEAP-Seq n confirming: | 2848 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | GGCAGTCGTGCTGATGTAGA |