Insertion junction: LMJ.RY0402.101054_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.101054
Chromosome:chromosome 1
Location:7518706
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g054150 NTRC1,NTR4 (1 of 1) PTHR10438//PTHR22912//PTHR22912:SF48 - THIOREDOXIN // DISULFIDE OXIDOREDUCTASE // NADPH-DEPENDENT THIOREDOXIN REDUCTASE 3; NADPH-dependent thioredoxin reductase C, chloroplastic intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCATCCCACCGTATAACTTCACCGCGAC
Internal bar code:CCTGAGGATGAGGGTATCGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:522
LEAP-Seq percent confirming:98.9005
LEAP-Seq n confirming:1709
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:AAACCTGTCGTGTTTGAGGG
Suggested primer 2:CCATACTTGCCTGGGTGTCT