| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.101059 |
| Chromosome: | chromosome 1 |
| Location: | 1430967 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g007200 | (1 of 1) K16675 - palmitoyltransferase ZDHHC9/14/18 (ZDHHC9_14_18) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAGGTATGCGGTCCATGCAGGGGGCTG |
| Internal bar code: | GCTAGTTAGTAACTCTTTCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1095 |
| LEAP-Seq percent confirming: | 98.1571 |
| LEAP-Seq n confirming: | 3249 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATTGAACGGTCCGAACTC |
| Suggested primer 2: | CCTACTCGCGCTACCTCAAC |