| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.101083 |
| Chromosome: | chromosome 1 |
| Location: | 1708506 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009101 | (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTGACAACTGAGTGCGCATGCAGTGAAT |
| Internal bar code: | GTACCTACGATGGCGAAATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 98.5555 |
| LEAP-Seq n confirming: | 1501 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGCTGTGCGAGTGAAAA |
| Suggested primer 2: | CAGCGAGACGTGAATGTTGT |