Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.101085 |
Chromosome: | chromosome 7 |
Location: | 2025864 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g325761 | (1 of 2) IPR000104//IPR003439//IPR003593//IPR011527//IPR027417 - Antifreeze protein, type I // ABC transporter-like // AAA+ ATPase domain // ABC transporter type 1, transmembrane domain // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCTTTCTTGGGAGTGGTGCCAAACAAAA |
Internal bar code: | GCAGGGCCCAGGGGTTGAGGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 375 |
LEAP-Seq percent confirming: | 98.5965 |
LEAP-Seq n confirming: | 281 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCATTCCAGAACCCAAAGC |
Suggested primer 2: | GAACCGGTATGGTGGGTATG |